быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
FADS1 Rabbit mAb (A0178)
- Описание
- Характеристики
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Overview
Product name FADS1 Rabbit mAb Catalog No. A0178 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2499 Background
The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 50-150 of human FADS1 (O60427). Sequence RRHPGGSRVISHYAGQDATDPFVAFHINKGLVKKYMNSLLIGELSPEQPSFEPTKNKELTDEFRELRATVERMGLMKANHVFFLLYLLHILLLDGAAWLTL Gene ID Swiss Prot Synonyms D5D; TU12; FADS6; FADSD5; LLCDL1 Calculated MW 52kDa Observed MW 52kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, Mouse liver, Rat lung, Rat liver, Rat brain Cellular location mitochondrion 
Western blot analysis of extracts of various cell lines, using FADS1 antibody (A0178) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 50-150 of human FADS1 (O60427). Isotype IgG