быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
EXOSC7 Rabbit mAb (A0341)
- Описание
- Характеристики
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Overview
Product name EXOSC7 Rabbit mAb Catalog No. A0341 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2501 Background
Predicted to enable 3'-5'-exoribonuclease activity and RNA binding activity. Predicted to be involved in RNA metabolic process. Part of exosome (RNase complex).Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 192-291 of human EXOSC7 (Q15024). Sequence LSVENVPCIVTLCKIGYRHVVDATLQEEACSLASLLVSVTSKGVVTCMRKVGKGSLDPESIFEMMETGKRVGKVLHASLQSVVHKEESLGPKRQKVGFLG Gene ID Swiss Prot Synonyms p8; EAP1; RRP42; Rrp42p; hRrp42p Calculated MW 32kDa Observed MW 37kDa Applications
Reactivity Human Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HepG2, A-431, K-562, U-251MG Cellular location Cytoplasm, Nucleus, nucleolus 
Western blot analysis of various lysates, using EXOSC7 antibody (A0341) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using EXOSC7 Rabbit mAb (A0341) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic peptide corresponding to a sequence within amino acids 192-291 of human EXOSC7 (Q15024). Isotype IgG