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Размер
- 100 μL
- 200 μL
- 50 μL
Collagen III alpha 1/COL3A1 Rabbit mAb (A0817)
- Описание
- Характеристики
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Overview
Product name Collagen III alpha 1/COL3A1 Rabbit mAb Catalog No. A0817 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2542 Background
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 150-400 of human Collagen III alpha 1/COL3A1 (P02461). Sequence NYSPQYDSYDVKSGVAVGGLAGYPGPAGPPGPPGPPGTSGHPGSPGSPGYQGPPGEPGQAGPSGPPGPPGAIGPSGPAGKDGESGRPGRPGERGLPGPPGIKGPAGIPGFPGMKGHRGFDGRNGEKGETGAPGLKGENGLPGENGAPGPMGPRGAPGERGRPGLPGAAGARGNDGARGSDGQPGPPGPPGTAGFPGSPGAKGEVGPAGSPGSNGAPGQRGEPGPQGHAGAQGPPGPPGINGSPGGKGEMGP Gene ID Swiss Prot Synonyms EDS4A; EDSVASC; PMGEDSV Calculated MW 139kDa Observed MW 150kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples HepG2, RD, SH-SY5Y, Mouse brain, Mouse uterus, Rat lung, Rat uterus Cellular location Secreted, extracellular matrix, extracellular space Customer validation WB(Homo sapiens, Mus musculus, Neurospora)
IHC(Rattus norvegicus)
Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (A0817) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (A0817) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Confocal imaging of HeLa cells using Collagen III alpha 1/COL3A1 Rabbit mAb (A0817, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Immunoprecipitation analysis of 300 μg extracts from HepG2 cells using 3 μg Collagen III alpha 1/COL3A1 Rabbit mAb (A0817). Western blot was performed from the immunoprecipitate using Collagen III alpha 1/COL3A1 Rabbit mAb (A0817) at a dilution of 1:1000.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 150-400 of human Collagen III alpha 1/COL3A1 (P02461). Isotype IgG