быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
ERK1 / ERK2 Mouse mAb (A10613)
- Описание
- Характеристики
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Overview
Product name ERK1 / ERK2 Mouse mAb Catalog No. A10613 Host species Mouse Purification method Affinity purification Isotype IgG CloneNo. AMC0488 Background
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.Immunogen information
Immunogen A synthetic peptide of human ERK1 / ERK2 Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW 36kDa/41kDa/38kDa/40kDa/43kDa Observed MW 42kDa/44kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, 293T, A-431, NIH/3T3, RAW 264.7, C6, PC12 Cellular location caveola, cytoplasm, cytoskeleton, cytosol, early endosome, endoplasmic reticulum lumen, extracellular region, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, mitotic spindle, nucleoplasm, nucleus, plasma membrane Customer validation WB(mouse cells, Homo sapiens, Mus musculus, Rattus norvegicus, Mouse sapiens )
IHC(Mus musculus)
IF(Mus musculus)
IP(Homo sapiens)
Western blot analysis of various lysates, using ERK1 / ERK2 Mouse mAb (A10613) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
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Key applications Western blotting Host species Мышь Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide of human ERK1 / ERK2 Isotype IgG