быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD34 Mouse mAb (A10798)
- Описание
- Характеристики
-
Overview
Product name CD34 Mouse mAb Catalog No. A10798 Host species Mouse Purification method Affinity purification Isotype IgG2b, kappa CloneNo. AMC0096 Background
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 32-290 of human CD34 (P28906). Sequence SLDNNGTATPELPTQGTFSNVSTNVSYQETTTPSTLGSTSLHPVSQHGNEATTNITETTVKFTSTSVITSVYGNTNSSVQSQTSVISTVFTTPANVSTPETTLKPSLSPGNVSDLSTTSTSLATSPTKPYTSSSPILSDIKAEIKCSGIREVKLTQGICLEQNKTSSCAEFKKDRGEGLARVLCGEEQADADAGAQVCSLLLAQSEVRPQCLLLVLANRTEISSKLQLMKKHQSDLKKLGILDFTEQDVASHQSYSQKT Gene ID Swiss Prot Synonyms CD34; CD34 molecule; GIG3; MORT1 Calculated MW 41kDa Observed MW 120kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, A-549 Cellular location Membrane, Single-pass type I membrane protein 
Western blot analysis of extracts of various cell lines, using CD34 antibody (A10798) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Мышь Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 32-290 of human CD34 (P28906). Isotype IgG2b, kappa