быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
MMP11 Rabbit mAb (A11088)
- Описание
- Характеристики
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Overview
Product name MMP11 Rabbit mAb Catalog No. A11088 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0523 Background
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is activated intracellularly by furin within the constitutive secretory pathway. Also in contrast to other MMP's, this enzyme cleaves alpha 1-proteinase inhibitor but weakly degrades structural proteins of the extracellular matrix.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 389-488 of human MMP11 (P24347). Sequence AALVWGPEKNKIYFFRGRDYWRFHPSTRRVDSPVPRRATDWRGVPSEIDAAFQDADGYAYFLRGRLYWKFDPVKVKALEGFPRLVGPDFFGCAEPANTFL Gene ID Swiss Prot Synonyms ST3; SL-3; STMY3 Calculated MW 55kDa Observed MW 55kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, A549, MCF7, Mouse lung, Mouse testis, Mouse kidney, Rat testis Cellular location Secreted, Extracellular matrix, Extracellular space Customer validation WB(Homo sapiens)
Western blot analysis of extracts of various cell lines, using MMP11 Rabbit mAb (A11088) at 1：1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 389-488 of human MMP11 (P24347). Isotype IgG