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Размер
- 100 μL
- 200 μL
- 50 μL
Glycophorin C (GYPC) Rabbit mAb (A11472)
- Описание
- Характеристики
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Overview
Product name Glycophorin C (GYPC) Rabbit mAb Catalog No. A11472 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0605 Background
Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 49-128 of human Glycophorin C (GYPC) (P04921). Sequence METSTPTIMDIVVIAGVIAAVAIVLVSLLFVMLRYMYRHKGTYHTNEAKGTEFAESADAALQGDPALQDAGDSSRKEYFI Gene ID Swiss Prot Synonyms GE; GPC; GPD; GYPD; CD236; PAS-2; CD236R; PAS-2' Calculated MW 14kDa Observed MW 30-40kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples K-562, Mouse liver, Rat liver Cellular location Cell membrane, Single-pass type III membrane protein 
Western blot analysis of extracts of various cell lines, using Glycophorin C (GYPC) Rabbit mAb (A11472) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from K-562 cells, using Glycophorin C (GYPC) Rabbit mAb (A11472) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human placenta using Glycophorin C (GYPC) (GYPC) Rabbit mAb (A11472) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 49-128 of human Glycophorin C (GYPC) (P04921). Isotype IgG