быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)
- Описание
- Характеристики
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Overview
Product name Monoamine Oxidase B (MAOB) Rabbit mAb Catalog No. A11597 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0641 Background
The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Monoamine Oxidase B (MAOB) (P27338). Sequence MSNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDRVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFR Gene ID Swiss Prot Synonyms MAOB; monoamine oxidase B Calculated MW 59kDa Observed MW 59kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, Hep G2 Cellular location Cytoplasmic side, Mitochondrion outer membrane, Single-pass type IV membrane protein 
Western blot analysis of extracts of various cell lines, using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded rat lung using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Monoamine Oxidase B (MAOB) (P27338). Isotype IgG