быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Glucagon Rabbit mAb (A11767)
- Описание
- Характеристики
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Overview
Product name Glucagon Rabbit mAb Catalog No. A11767 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0382 Background
The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.Immunogen information
Immunogen A synthesized peptide derived from human Glucagon Sequence Email for sequence Gene ID Swiss Prot Synonyms GLP1; GLP2; GRPP; GLP-1 Calculated MW 21kDa Observed MW Refer to figures Applications
Reactivity Mouse, Rat Tested applications IHC-P IF/ICC Recommended dilution - IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Immunohistochemistry Immunofluorescence Positive samples Cellular location endoplasmic reticulum lumen, extracellular region, extracellular space 
Western blot analysis of extracts of Rat pancreas cells, using Glucagon antibody (A11767) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using Glucagon Rabbit mAb (A11767) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse pancreatic islet using Glucagon Rabbit mAb (A11767) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat pancreas using Glucagon Rabbit mAb (A11767) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse pancreas using Glucagon Rabbit mAb (A11767) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen A synthesized peptide derived from human Glucagon Isotype IgG