быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
NeuroD1 Rabbit mAb (A12020)
- Описание
- Характеристики
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Overview
Product name NeuroD1 Rabbit mAb Catalog No. A12020 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0483 Background
This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus.Immunogen information
Immunogen A synthesized peptide derived from human NeuroD1. Sequence Email for sequence Gene ID Swiss Prot Synonyms T2D; BETA2; BHF-1; MODY6; NEUROD; bHLHa3 Calculated MW 40kDa Observed MW 45kDa Applications
Reactivity Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples Mouse brain Cellular location Cytoplasm, Nucleoplasm, Nucleus 
Western blot analysis of extracts of mouse brain, using NeuroD1 Rabbit mAb (A12020) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen A synthesized peptide derived from human NeuroD1. Isotype IgG