быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
PI3 Kinase p110 beta Rabbit mAb (A1271)
- Описание
- Характеристики
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Overview
Product name PI3 Kinase p110 beta Rabbit mAb Catalog No. A1271 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0703 Background
This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen A synthesized peptide derived from human PI3 Kinase p110 beta Sequence Email for sequence Gene ID Swiss Prot Synonyms PI3K; PIK3C1; P110BETA; PI3KBETA Calculated MW 123kDa Observed MW 110kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples A-549, MCF7 Cellular location cytoplasm, cytosol, nucleolus, nucleoplasm, nucleus, plasma membrane 
Western blot analysis of extracts of various cell lines, using PI3 Kinase p110 beta antibody (A1271) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthesized peptide derived from human PI3 Kinase p110 beta Isotype IgG