быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
BDNF Mouse mAb (A18129)
- Описание
- Характеристики
-
Overview
Product name BDNF Mouse mAb Catalog No. A18129 Host species Mouse Purification method Affinity purification Isotype lgG2a, kappa CloneNo. AMC0122 Background
This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-247 of human BDNF (P23560). Sequence PMKEANIRGQGGLAYPGVRTHGTLESVNGPKAGSRGLTSLADTFEHVIEELLDEDQKVRPNEENNKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR Gene ID Swiss Prot Synonyms ANON2; BULN2 Calculated MW 28kDa Observed MW 28kDa Applications
Reactivity Mouse, Rat Tested applications WB Recommended dilution - WB 1:2000 - 1:10000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples Mouse lung, Mouse brain, Rat lung Cellular location Secreted Customer validation WB(Mus musculus)
IHC(Mus musculus)
Western blot analysis of extracts of various cell lines, using BDNF Mouse mAb antibody (A18129) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Мышь Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 20-247 of human BDNF (P23560). Isotype lgG2a, kappa