быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
HDAC1 Mouse mAb (A18304)
- Описание
- Характеристики
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Overview
Product name HDAC1 Mouse mAb Catalog No. A18304 Host species Mouse Purification method Affinity purification Isotype IgG1, Kappa CloneNo. AMC0232 Background
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (Q13547). Sequence SGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA Gene ID Swiss Prot Synonyms Calculated MW Observed MW 62kDa Applications
Reactivity Human, Mouse Tested applications WB IHC-P IP ChIP Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
- ChIP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, 293T, BxPC-3 Cellular location Customer validation WB、IHC 、IF(Homo sapiens、Mus musculus)
Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (A18304) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal using HDAC1 Mouse mAb (A18304) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Chromatin immunoprecipitation analysis of extracts of K-562 cells, using HDAC1 antibody (A18304) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Key applications Western blotting, Immunohistochemistry Host species Мышь Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (Q13547). Isotype IgG1, Kappa