быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Neurofilament M Rabbit mAb (A19085)
- Описание
- Характеристики
-
Overview
Product name Neurofilament M Rabbit mAb Catalog No. A19085 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0396 Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 600-700 of human Neurofilament M (P07197). Sequence ELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGE Gene ID Swiss Prot Synonyms NFM; NEF3; NF-M Calculated MW 102kDa Observed MW 160kDa Applications
Reactivity Human Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T, SH-SY5Y Cellular location axon, cytoplasm, intermediate filament cytoskeleton, neurofilament, postsynaptic intermediate filament cytoskeleton 
Western blot analysis of extracts of various cell lines, using Neurofilament M antibody (A19085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of SH-SY5Y cells using Neurofilament M Rabbit mAb (A19085) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
-
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen A synthetic peptide corresponding to a sequence within amino acids 600-700 of human Neurofilament M (P07197). Isotype IgG