быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CDKN1B/p27KIP1 Rabbit mAb (A19095)
- Описание
- Характеристики
-
Overview
Product name CDKN1B/p27KIP1 Rabbit mAb Catalog No. A19095 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0455 Background
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4).Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 99-198 of human CDKN1B/p27KIP1 (P46527). Sequence CKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT Gene ID Swiss Prot Synonyms KIP1; MEN4; CDKN4; MEN1B; P27KIP1 Calculated MW 22kDa Observed MW 27kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HepG2 cells, 293T cells, C6 cells, HeLa cells, Mouse lung, Rat lung Cellular location Cytoplasm, Endosome, Nucleus Customer validation WB(Homo sapiens, Homo sapiens，Rattus norvegicus)
Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 antibody (A19095) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
Immunohistochemistry of paraffin-embedded human brain using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human breast cancer using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human lung adenocarcinoma using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human placenta using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human spleen using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human thyroid using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human tonsil using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat ovary using CDKN1B/p27KIP1 Rabbit mAb (A19095) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
-
Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 99-198 of human CDKN1B/p27KIP1 (P46527). KO Validated Validated Isotype IgG