быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Paxillin Rabbit mAb (A19100)
- Описание
- Характеристики
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Overview
Product name Paxillin Rabbit mAb Catalog No. A19100 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0368 Background
This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445).Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Paxillin (P49023). Sequence MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNP Gene ID Swiss Prot Synonyms Paxillin; PXN; paxillin Calculated MW 65kDa/61kDa/66kDa/47kDa Observed MW 65kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, PC-3, NIH/3T3, Mouse lung, Rat testis, Cellular location Cell junction, Cytoplasm, cell cortex, cytoskeleton, focal adhesion 
Western blot analysis of extracts of various cell lines, using Paxillin antibody (A19100) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of HeLa cells, using Paxillin antibody (A19100) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Paxillin (P49023). Isotype IgG