быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
HOXA9 Rabbit mAb (A19257)
- Описание
- Характеристики
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Overview
Product name HOXA9 Rabbit mAb Catalog No. A19257 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2423 Background
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. This gene is highly similar to the abdominal-B (Abd-B) gene of Drosophila. A specific translocation event which causes a fusion between this gene and the NUP98 gene has been associated with myeloid leukemogenesis. Read-through transcription exists between this gene and the upstream homeobox A10 (HOXA10) gene.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 173-272 of human HOXA9 (P31269). Sequence GAFSENNAENESGGDKPPIDPNNPAANWLHARSTRKKRCPYTKHQTLELEKEFLFNMYLTRDRRYEVARLLNLTERQVKIWFQNRRMKMKKINKDRAKDE Gene ID Swiss Prot Synonyms HOX1; ABD-B; HOX1G; HOX1.7 Calculated MW 30kDa Observed MW 30kDa Applications
Reactivity Human, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Rat large intestine, Rat liver Cellular location Nucleus 
Western blot analysis of extracts of various cell lines, using HOXA9 antibody (A19257) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human placenta using HOXA9 Rabbit mAb (A19257) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 173-272 of human HOXA9 (P31269). Isotype IgG