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Размер
- 100 μL
- 200 μL
- 50 μL
Cytokeratin 12 (KRT12) Rabbit mAb (A19284)
- Описание
- Характеристики
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Overview
Product name Cytokeratin 12 (KRT12) Rabbit mAb Catalog No. A19284 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2465 Background
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 250-494 of human Cytokeratin 12 (KRT12) (NP_000214.1). Sequence EMQIESLNEELAYMKKNHEDELQSFRVGGPGEVSVEMDAAPGVDLTRLLNDMRAQYETIAEQNRKDAEAWFIEKSGELRKEISTNTEQLQSSKSEVTDLRRAFQNLEIELQSQLAMKKSLEDSLAEAEGDYCAQLSQVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKARLELEIETYRRLLDGEAQGDGLEESLFVTDSKSQAQSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEIEELM Gene ID Swiss Prot Synonyms K12; MECD1 Calculated MW 54kDa Observed MW 54kDa Applications
Reactivity Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:100 - 1:500
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Rat eye Cellular location Cytoskeleton, Cytosol, Extracellular exosome 
Western blot analysis of extracts of Rat eye, using Cytokeratin 12 (KRT12) (KRT12) antibody (A19284) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded rat cornea using Cytokeratin 12 (KRT12) (KRT12) Rabbit mAb (A19284) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded Mouse cornea using Cytokeratin 12 (KRT12) (KRT12) Rabbit mAb (A19284) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of Mouse eye using Cytokeratin 12 (KRT12) antibody (A19284) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Rat eye using Cytokeratin 12 (KRT12) antibody (A19284) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 250-494 of human Cytokeratin 12 (KRT12) (NP_000214.1). Isotype IgG