быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
TCF4/TCF7L2 Rabbit mAb (A19548)
- Описание
- Характеристики
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Overview
Product name TCF4/TCF7L2 Rabbit mAb Catalog No. A19548 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0027 Background
This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TCF4/TCF7L2 (Q9NQB0). Sequence MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPY Gene ID Swiss Prot Synonyms TCF4; TCF-4 Calculated MW 68kDa Observed MW 68kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples SW620, HeLa, U-251MG, HepG2 Cellular location nucleoplasm, nucleus, PML body Customer validation WB(Mus musculus, Rattus norvegicus)
Western blot analysis of extracts of various cell lines, using TCF4/TCF4/TCF7L2 antibody (A19548) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat testis using TCF4/TCF4/TCF7L2 antibody (A19548) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using TCF4/TCF4/TCF7L2 antibody (A19548) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using TCF4/TCF4/TCF7L2 antibody (A19548) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TCF4/TCF7L2 (Q9NQB0). Isotype IgG