быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Hsp90β Rabbit mAb (A19574)
- Описание
- Характеристики
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Overview
Product name Hsp90β Rabbit mAb Catalog No. A19574 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0053 Background
This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90β (NP_031381.2). Sequence MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEE Gene ID Swiss Prot Synonyms HSP84; HSPC2; HSPCB; D6S182; HSP90B Calculated MW 83kDa Observed MW 85kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, A-431, 293T, Mouse kidney, Mouse brain, Mouse liver, Rat heart, Rat testis, Rat liver Cellular location Cytoplasm, Melanosome Customer validation ( 293T HelaEGF HepG2 A549 Remas 扁桃体 Jurket )
IF(Homo sapiens)
Western blot analysis of extracts of various cell lines, using Hsp90β antibody (A19574) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.
Immunofluorescence analysis of C6 cells using Hsp90β antibody (A19574). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Hsp90β antibody (A19574). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Hsp90β antibody (A19574). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90β (NP_031381.2). Isotype IgG