быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Calsequestrin 1 Rabbit mAb (A19640)
- Описание
- Характеристики
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Overview
Product name Calsequestrin 1 Rabbit mAb Catalog No. A19640 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2209 Background
This gene encodes the skeletal muscle specific member of the calsequestrin protein family. Calsequestrin functions as a luminal sarcoplasmic reticulum calcium sensor in both cardiac and skeletal muscle cells. This protein, also known as calmitine, functions as a calcium regulator in the mitochondria of skeletal muscle. This protein is absent in patients with Duchenne and Becker types of muscular dystrophy.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Calsequestrin 1 (NP_001222.3). Sequence EDKGVGFGLVDSEKDAAVAKKLGLTEVDSMYVFKGDEVIEYDGEFSADTIVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGYFKSKDSEHYKAFEDA Gene ID Swiss Prot Synonyms CASQ; CSQ1; PDIB1; VMCQA Calculated MW 45kDa Observed MW 55kDa Applications
Reactivity Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples Mouse skeletal muscle, Rat heart Cellular location Endoplasmic reticulum, Mitochondrial matrix 
Western blot analysis of extracts of Rat heart, using Calsequestrin 1 Rabbit mAb (A19640) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot analysis of Mouse skeletal muscle, using Calsequestrin 1 Rabbit mAb (A19640) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunofluorescence analysis of Mouse skeletal muscle using Calsequestrin 1 antibody (A19640) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Rat skeletal muscle using Calsequestrin 1 antibody (A19640) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human Calsequestrin 1 (NP_001222.3). Isotype IgG