быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
EDG2/LPA-1 Rabbit mAb (A19671)
- Описание
- Характеристики
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Overview
Product name EDG2/LPA-1 Rabbit mAb Catalog No. A19671 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2224 Background
The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling, EDG receptors mediate diverse biologic functions, including proliferation, platelet aggregation, smooth muscle contraction, inhibition of neuroblastoma cell differentiation, chemotaxis, and tumor cell invasion. Many transcript variants encoding a few different isoforms have been identified for this gene.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EDG2/LPA-1 (Q92633). Sequence MAAISTSIPVISQPQFTAMNEPQCFYNESIAFFYNRSGKHLATEWNTVSKLVMGLGITVCIFIMLANLLVMVAIYVNRRFHFPIYYLMANLAAADFFAGL Gene ID Swiss Prot Synonyms EDG2; LPA1; VZG1; edg-2; vzg-1; Gpcr26; Mrec1.3; rec.1.3 Calculated MW 41kDa Observed MW 41kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, Jurkat, Mouse lung, Mouse brain, Rat brain Cellular location cytoplasm, dendritic shaft, dendritic spine, endosome, GABA-ergic synapse, glutamatergic synapse, plasma membrane, postsynaptic membrane, presynaptic membrane 
Western blot analysis of extracts of various cell lines, using EDG2/LPA-1 Rabbit mAb (A19671) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EDG2/LPA-1 (Q92633). Isotype IgG