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Размер
- 100 μL
- 200 μL
- 50 μL
Cytokeratin 18 (KRT18) Rabbit mAb (A19778)
- Описание
- Характеристики
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Overview
Product name Cytokeratin 18 (KRT18) Rabbit mAb Catalog No. A19778 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2303 Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 250-430 of human Cytokeratin 18 (KRT18) (P05783). Sequence ADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH Gene ID Swiss Prot Synonyms K18; CK-18; CYK18 Calculated MW 48kDa Observed MW 48kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples HeLa, HepG2, A-431 Cellular location adherens junction, cytoplasm, cytoskeleton, cytosol, extracellular exosome, microtubule organizing center, nuclear matrix, nucleolus, perinuclear region of cytoplasm Customer validation IF(Rattus norvegicus)
IHC(Homo sapiens )
Western blot analysis of extracts of various cell lines, using Cytokeratin 18 (KRT18) Rabbit mAb (A19778) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast cancer using Cytokeratin 18 (KRT18) Rabbit mAb (A19778) at dilution of 1:800 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human colon carcinoma using Cytokeratin 18 (KRT18) Rabbit mAb (A19778) at dilution of 1:800 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human colon using Cytokeratin 18 (KRT18) Rabbit mAb (A19778) at dilution of 1:800 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver using Cytokeratin 18 (KRT18) Rabbit mAb (A19778) at dilution of 1:800 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of Human mammary cancer using Cytokeratin 18 (KRT18) antibody (A19778) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Mouse colon using Cytokeratin 18 (KRT18) antibody (A19778) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Rat rectum using Cytokeratin 18 (KRT18) antibody (A19778) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 250-430 of human Cytokeratin 18 (KRT18) (P05783). Isotype IgG