быстрая доставка ~4 недели
Размер
- 100 μL
- 50 μL
IL2 Rabbit mAb (A20224)
- Описание
- Характеристики
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Overview
Product name IL2 Rabbit mAb Catalog No. A20224 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC5130-01 Background
This gene is a member of the interleukin 2 (IL2) cytokine subfamily which includes IL4, IL7, IL9, IL15, IL21, erythropoietin, and thrombopoietin. The protein encoded by this gene is a secreted cytokine produced by activated CD4+ and CD8+ T lymphocytes, that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2R) is a heterotrimeric protein complex whose gamma chain is also shared by IL4 and IL7. The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.Immunogen information
Immunogen Recombinant protein of human IL2. Sequence Email for sequence Gene ID Swiss Prot Synonyms IL-2; TCGF; lymphokine Calculated MW 18kDa Observed MW 14kDa/15kDa Applications
Reactivity Human Tested applications WB FC ELISA Recommended dilution - Elisa 1:500 - 1:2000
- WB 1:500 - 1:1000
- FC 1:50 - 1:200
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% thimerosal, pH7.3.Key application Western blotting Flow Cytometry Positive samples Recombinant Human IL-2 Protein, Jurkat+TPA+A23187+Brefeldin A Cellular location Secreted 
Western blot analysis of extracts of 293T cells, using IL2 antibody (A20224) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts of Jurkat cells, using IL2 antibody (A20224) at 1:1000 dilution.Jurkat cells were treated by TPA (40 nM), A23187 (2μM) and Brefeldin A (300 ng / ml) for 0-24hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Flow cytometry was performed with Jurkat cells.
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Key applications Western blotting, Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human IL2. Isotype IgG