быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Neurofilament L Rabbit mAb (A20269)
- Описание
- Характеристики
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Overview
Product name Neurofilament L Rabbit mAb Catalog No. A20269 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC50056 Background
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human Neurofilament L (NP_006149.2). Sequence SSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVT Gene ID Swiss Prot Synonyms NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110 Calculated MW 62kDa Observed MW 70kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples SH-SY5Y Cellular location axon, axon cytoplasm, cholinergic synapse, cytoplasm, cytosol, growth cone, neurofilament, neuromuscular junction, postsynaptic intermediate filament cytoskeleton, presynaptic intermediate filament cytoskeleton, Schaffer collateral - CA1 synapse 
Western blot analysis of extracts from SH-SY5Y cells, using Neurofilament L antibody (A20269) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Neurofilament L Rabbit mAb (A20269) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using Neurofilament L Rabbit mAb (A20269) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal imaging of human brain using Neurofilament L Rabbit mAb (A20269, dilution 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human Neurofilament L (NP_006149.2). Isotype IgG