быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
MLH1 Mouse mAb (A20544)
- Описание
- Характеристики
-
Overview
Product name MLH1 Mouse mAb Catalog No. A20544 Host species Mouse Purification method Affinity purification Isotype IgG1, Kappa CloneNo. AMC0417 Background
The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).Immunogen information
Immunogen Recombinant protein of human MLH1. Sequence Email for sequence Gene ID Swiss Prot Synonyms FCC2; COCA2; HNPCC; MLH-1; hMLH1; HNPCC2; LYNCH2; MMRCS1 Calculated MW Observed MW 85kDa Applications
Reactivity Human, Mouse Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Jurkat, HeLa Cellular location Nucleus Customer validation IHC(Homo sapiens)
Immunohistochemistry analysis of paraffin-embedded human appendix using MLH1 Mouse mAb (A20544) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MLH1 Mouse mAb (A20544) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human pancreas using MLH1 Mouse mAb (A20544) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
-
Key applications Western blotting, Immunohistochemistry Host species Мышь Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of human MLH1. KO Validated Validated Isotype IgG1, Kappa