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Размер
- 100 μL
- 200 μL
- 50 μL
GSDMD Rabbit mAb (A20728)
- Описание
- Характеристики
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Overview
Product name GSDMD Rabbit mAb Catalog No. A20728 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC50993 Background
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 346-484 of human GSDMD (NP_079012.3). Sequence DGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH Gene ID Swiss Prot Synonyms DF5L; DFNA5L; FKSG10; GSDMDC1 Calculated MW 53kDa Observed MW 53kDa/22kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples THP-1, A-431 Cellular location cytosol, extracellular region, extracellular space, NLRP3 inflammasome complex, nucleoplasm, plasma membrane Customer validation WB(Mus musculus, Homo sapiens, Rattus norvegicus)
IHC(Homo sapiens)
Western blot analysis of extracts of A-431 cells, using GSDMD antibody (A20728) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Western blot analysis of extracts of THP-1, using GSDMD antibody (A20728) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 346-484 of human GSDMD (NP_079012.3). Isotype IgG