быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Myeloperoxidase (MPO) Mouse mAb (A20748)
- Описание
- Характеристики
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Overview
Product name Myeloperoxidase (MPO) Mouse mAb Catalog No. A20748 Host species Mouse Purification method Affinity purification Isotype IgG1 CloneNo. AMC0493 Background
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.Immunogen information
Immunogen Recombinant protein of human Myeloperoxidase (MPO). Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 60kDa Applications
Reactivity Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples Rat lung Cellular location 
Western blot analysis of extracts of Rat lung, using Myeloperoxidase (MPO) antibody (A20748) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Мышь Antibody type Primary Antibodies Reactivity Крыса Immunogen Recombinant protein of human Myeloperoxidase (MPO). Isotype IgG1