быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
P2Y12 Rabbit mAb (A20864)
- Описание
- Характеристики
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Overview
Product name P2Y12 Rabbit mAb Catalog No. A20864 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2792 Background
Enables G protein-coupled ADP receptor activity and G protein-coupled adenosine receptor activity. Involved in several processes, including cellular response to ATP; regulation of microglial cell migration; and substrate-dependent cell migration, cell extension. Acts upstream of or within several processes, including adenylate cyclase-modulating G protein-coupled receptor signaling pathway; platelet activation; and positive regulation of ion transport. Located in cell body membrane and cell projection membrane. Is expressed in central nervous system and cerebral cortex. Used to study platelet-type bleeding disorder 8. Human ortholog(s) of this gene implicated in asthma; cerebrovascular disease; peripheral artery disease; platelet-type bleeding disorder 8; and type 2 diabetes mellitus. Orthologous to human P2RY12 (purinergic receptor P2Y12).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 120-347 of mouse Recombinant protein of Mouse P2Y12 (Q9CPV9). Sequence FLGLITIDRYLKTTRPFKTSSPSNLLGAKILSVVIWAFMFLISLPNMILTNRRPKDKDVTKCSFLKSEFGLVWHEIVNYICQVIFWINFLIVIVCYSLITKELYRSYVRTRGSAKVPKKKVNVKVFIIIAVFFICFVPFHFARIPYTLSQTRAVFDCSAENTLFYVKESTLWLTSLNACLDPFIYFFLCKSFRNSLTSMLRCSNSTSTSGTNKKKGQEGGEPSEETPM Gene ID Swiss Prot Synonyms P2Y12; 2900079B22Rik; 4921504D23Rik Calculated MW 39kDa Observed MW 55kDa Applications
Reactivity Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples C6, Mouse brain, Mouse spinal cord, Rat brain Cellular location basal plasma membrane, caveola, cell body membrane, external side of plasma membrane, mitochondrion, plasma membrane 
Western blot analysis of extracts of various cell lines, using P2Y12 antibody (A20864) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunofluorescence analysis of Rat brain using P2Y12 antibody (A20864) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Mouse brain using P2Y12 antibody (A20864) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 120-347 of mouse Recombinant protein of Mouse P2Y12 (Q9CPV9). Isotype IgG