быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
HNF-4-alpha Rabbit mAb (A20865)
- Описание
- Характеристики
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Overview
Product name HNF-4-alpha Rabbit mAb Catalog No. A20865 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2794 Background
The protein encoded by this gene is a transcription factor involved in the development of the pancreas, liver, kidney, and intestines. The encoded protein also functions to maintain glucose homeostasis. Several transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of mouse Recombinant protein of Mouse HNF-4-alpha (P49698). Sequence MRLSKTLAGMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGANLNSSNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSQQITSPISGINGDIRAKKIANITDVCESMKEQLLVLVE Gene ID Swiss Prot Synonyms Hnf4; HNF-4; MODY1; Nr2a1; Tcf14; TCF-14; Hnf4alpha Calculated MW 53kDa Observed MW 53kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IP ChIP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- ChIP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Mouse liver, Rat liver Cellular location Nucleus 
Western blot analysis of extracts of various cell lines, using HNF-4-alpha antibody (A20865) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using HNF-4-alpha Rabbit mAb (A20865) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using HNF-4-alpha Rabbit mAb (A20865) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human small intestine using HNF-4-alpha Rabbit mAb (A20865) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using HNF-4-alpha antibody (A20865) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of mouse Recombinant protein of Mouse HNF-4-alpha (P49698). Isotype IgG