быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Importin 9 / RANBP9 Rabbit mAb (A20901)
- Описание
- Характеристики
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Overview
Product name Importin 9 / RANBP9 Rabbit mAb Catalog No. A20901 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2864 Background
Enables nuclear import signal receptor activity. Involved in protein import into nucleus. Located in cytoplasm.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Importin 9 / RANBP9 (Q96P70). Sequence LEVTEEFGVHLAELTVDPQGALAIRQLASVILKQYVETHWCAQSEKFRPPETTERAKIVIRELLPNGLRESISKVRSSVAYAVSAIAHWDWPEAWPQLFNL Gene ID Swiss Prot Synonyms Imp9 Calculated MW 116kDa Observed MW 116kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, Jurkat, U-251MG, Mouse testis, Rat testis, Rat brain, Mouse brain Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts of various cell lines, using Importin 9/RANBP9 antibody (A20901) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Importin 9 / RANBP9 Rabbit mAb (A20901) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Importin 9 / RANBP9 (Q96P70). Isotype IgG