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Размер
- 100 μL
- 200 μL
- 50 μL
MYO19 Rabbit mAb (A20937)
- Описание
- Характеристики
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Overview
Product name MYO19 Rabbit mAb Catalog No. A20937 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2919 Background
Enables actin binding activity. Involved in regulation of cytokinesis and regulation of mitochondrial fission. Acts upstream of or within mitochondrion migration along actin filament. Located in cytosol and mitochondrial outer membrane.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 450-650 of human MYO19 (Q96H55). Sequence QQEEYAVEGLEWSFINYQDNQPCLDLIEGSPISICSLINEECRLNRPSSAAQLQTRIETALAGSPCLGHNKLSREPSFIVVHYAGPVRYHTAGLVEKNKDPIPPELTRLLQQSQDPLLMGLFPTNPKEKTQEEPPGQSRAPVLTVVSKFKASLEQLLQVLHSTTPHYIRCIKPNSQGQAQTFLQEEVLSQLEACGLVETIH Gene ID Swiss Prot Synonyms MYOHD1 Calculated MW 109kDa Observed MW 109kDa Applications
Reactivity Human, Mouse, Rat, Monkey Tested applications WB Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, COS-7, Mouse thymus, Rat testis Cellular location Actin cytoskeleton, cytosol, mitochondrial outer membrane, mitochondrion 
Western blot analysis of extracts of Rat testis, using MYO19 antibody (A20937) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using MYO19 antibody (A20937) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Обезьяна Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 450-650 of human MYO19 (Q96H55). Isotype IgG