быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
TCEA1 Rabbit mAb (A20972)
- Описание
- Характеристики
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Overview
Product name TCEA1 Rabbit mAb Catalog No. A20972 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2962 Background
Predicted to enable DNA binding activity; translation elongation factor activity; and zinc ion binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II. Predicted to act upstream of or within erythrocyte differentiation and positive regulation of transcription, DNA-templated. Located in nucleolus and nucleoplasm. Part of transcription factor TFIID complex.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TCEA1 (P23193). Sequence VNAIRKQSTDEEVTSLAKSLIKSWKKLLDGPSTEKDLDEKKKEPAITSQNSPEAREESTSSGNVSNRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAA Gene ID Swiss Prot Synonyms SII; TCEA; TF2S; GTF2S; TFIIS Calculated MW 34kDa Observed MW 40kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:100 - 1:500
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples SP2/0, HeLa, K-562, Mouse liver, Mouse brain, Rat thymus Cellular location Nucleus 
Western blot analysis of extracts of various cell lines, using TCEA1 Rabbit mAb (A20972) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TCEA1 Rabbit mAb (A20972) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using TCEA1 Rabbit mAb (A20972) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using TCEA1 Rabbit mAb (A20972) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat ovary using TCEA1 Rabbit mAb (A20972) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using TCEA1 Rabbit mAb (A20972) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using TCEA1 Rabbit mAb (A20972) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 50-150 of human TCEA1 (P23193). Isotype IgG