быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Semaphorin 4D / CD100 Rabbit mAb (A21070)
- Описание
- Характеристики
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Overview
Product name Semaphorin 4D / CD100 Rabbit mAb Catalog No. A21070 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2801 Background
Enables identical protein binding activity; semaphorin receptor binding activity; and transmembrane signaling receptor activity. Involved in several processes, including positive regulation of phosphatidylinositol 3-kinase signaling; regulation of neuron projection development; and regulation of phosphate metabolic process. Is integral component of plasma membrane.Immunogen information
Immunogen Recombinant protein of Human Semaphorin 4D / CD100. Sequence Email for sequence Gene ID Swiss Prot Synonyms A8; GR3; BB18; CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G Calculated MW 96kDa Observed MW 140kDa Applications
Reactivity Human Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Daudi Cellular location Cell membrane 
Western blot analysis of extracts of various cell lines, using Semaphorin 4D / CD100 antibody (A21070) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human tonsil using Semaphorin 4D / CD100 Rabbit mAb (A21070) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of Human Semaphorin 4D / CD100. Isotype IgG