быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD32A + CD32B + CD32C Rabbit mAb (A21073)
- Описание
- Характеристики
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Overview
Product name CD32A + CD32B + CD32C Rabbit mAb Catalog No. A21073 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2905 Background
This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Sequence Email for sequence Gene ID Swiss Prot Synonyms CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1; FcgammaRIIa Calculated MW 35kDa/34kDa Observed MW 25-50kDa Applications
Reactivity Human Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Raji, Daudi, K-562, U-937, THP-1, HL-60, HLE Cellular location Golgi apparatus, plasma membrane 
Western blot analysis of extracts of various cell lines, using CD32A + CD32B + CD32C antibody (A21073) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human lung using CD32A + CD32B + CD32C Rabbit mAb (A21073) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using CD32A + CD32B + CD32C Rabbit mAb (A21073) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Isotype IgG