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Размер
- 100 μL
- 200 μL
- 50 μL
PLK1 Rabbit mAb (A21082)
- Описание
- Характеристики
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Overview
Product name PLK1 Rabbit mAb Catalog No. A21082 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC52311 Background
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 272-495 of human PLK1 (NP_005021.2). Sequence KHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGA Gene ID Swiss Prot Synonyms PLK; STPK13 Calculated MW 68kDa Observed MW 62kDa Applications
Reactivity Human, Mouse, Rat, Monkey Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, Jurkat, K-562, COS-7, Mouse testis, Rat testis Cellular location Chromosome, Cytoplasm, Midbody, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle Customer validation (胃肠间质瘤GIST)
Western blot analysis of extracts of various cell lines, using PLK1 antibody (A21082) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using PLK1 antibody (A21082) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Обезьяна Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 272-495 of human PLK1 (NP_005021.2). Isotype IgG
