быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Osteopontin Mouse mAb (A21084)
- Описание
- Характеристики
-
Overview
Product name Osteopontin Mouse mAb Catalog No. A21084 Host species Mouse Purification method Affinity purification Isotype IgG2a, Kappa CloneNo. AMC0443 Background
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of human Osteopontin. Sequence Email for sequence Gene ID Swiss Prot Synonyms OPN; BNSP; BSPI; ETA-1 Calculated MW 35kDa Observed MW 69kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, HepG2, U-87MG, C2C12, Mouse bone, Rat kidney Cellular location Secreted 
Western blot analysis of extracts of various cell lines, using Osteopontin antibody (A21084) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Мышь Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human Osteopontin. Isotype IgG2a, Kappa