быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
Caspase-1 Rat mAb (A21085)
- Описание
- Характеристики
-
Overview
Product name Caspase-1 Rat mAb Catalog No. A21085 Host species Rat Purification method Affinity purification Isotype IgG CloneNo. ATC0001 Background
Enables cysteine-type endopeptidase activity. Involved in several processes, including positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-1 beta production; and protein autoprocessing. Acts upstream of or within several processes, including membrane hyperpolarization; mitochondrial depolarization; and positive regulation of interleukin-1 alpha production. Located in extracellular region. Part of IPAF inflammasome complex. Is expressed in knee joint and metanephros. Orthologous to human CASP1 (caspase 1).Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 206-220aa of mouse Caspase-1. Sequence TALEMVKEVKEFAAC Gene ID Swiss Prot Synonyms ICE; Il1bc Calculated MW 46kDa Observed MW 48kDa/33kDa Applications
Reactivity Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples Mouse lung, Mouse spleen, Rat lung Cellular location Cytoplasm 
Western blot analysis of extracts of various cell lines, using Caspase-1 antibody (A21085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rat IgG (H+L) (AS028) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Крыса Antibody type Primary Antibodies Reactivity Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 206-220aa of mouse Caspase-1. Isotype IgG