быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
MYO1B Rabbit mAb (A21113)
- Описание
- Характеристики
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Overview
Product name MYO1B Rabbit mAb Catalog No. A21113 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2990 Background
Enables ATP binding activity; actin filament binding activity; and microfilament motor activity. Involved in actin filament organization and post-Golgi vesicle-mediated transport. Located in several cellular components, including actin filament; endosome; and perinuclear region of cytoplasm. Colocalizes with trans-Golgi network membrane.Immunogen information
Immunogen Recombinant protein of Human MYO1B. Sequence Email for sequence Gene ID Swiss Prot Synonyms MMIa; myr1; MYH-1c; MMI-alpha Calculated MW 132kDa Observed MW 132kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples A-431, Mouse lung, Rat lung Cellular location actin cytoskeleton, cytoplasm, early endosome, extracellular exosome, filopodium, perinuclear region of cytoplasm, plasma membrane 
Western blot analysis of extracts of Mouse lung, using MYO1B antibody (A21113) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using MYO1B antibody (A21113) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of Human MYO1B. Isotype IgG