быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
PRD Rabbit mAb (A21120)
- Описание
- Характеристики
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Overview
Product name PRD Rabbit mAb Catalog No. A21120 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3002 Background
This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of Human PRD. Sequence Email for sequence Gene ID Swiss Prot Synonyms PROLIDASE Calculated MW 55kDa Observed MW 60kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, HepG2, SH-SY5Y, Mouse small intestine Cellular location extracellular exosome 
Western blot analysis of extracts of various cell lines, using PRD antibody (A21120) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of Mouse small intestine, using PRD antibody (A21120) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of Human PRD. Isotype IgG