быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
PP2A-B56δ/PR61δ/PPP2R5D Rabbit mAb (A21122)
- Описание
- Характеристики
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Overview
Product name PP2A-B56δ/PR61δ/PPP2R5D Rabbit mAb Catalog No. A21122 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC2981 Background
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.Immunogen information
Immunogen Recombinant protein of Human PPP2R5D. Sequence Email for sequence Gene ID Swiss Prot Synonyms B56D; MRD35; B56delta Calculated MW 70kDa Observed MW 66kDa/69kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HepG2, Mouse lung, Mouse brain, Rat testis, Rat brain Cellular location Cytoplasm, Nucleus 
Western blot analysis of various lysates, using PP2A-B56δ/PR61δ/PPP2R5D antibody (A21122) at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using PP2A-B56δ/PR61δ/PPP2R5D Rabbit mAb (A21122) at dilution of 1:20(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using PP2A-B56δ/PR61δ/PPP2R5D Rabbit mAb (A21122) at dilution of 1:20(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse heart using PP2A-B56δ/PR61δ/PPP2R5D Rabbit mAb (A21122) at dilution of 1:20(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat ovary using PP2A-B56δ/PR61δ/PPP2R5D Rabbit mAb (A21122) at dilution of 1:20(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of Human PPP2R5D. Isotype IgG