быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
SLC25A12 Rabbit mAb (A21129)
- Описание
- Характеристики
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Overview
Product name SLC25A12 Rabbit mAb Catalog No. A21129 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3041 Background
This gene encodes a calcium-binding mitochondrial carrier protein. The encoded protein localizes to the mitochondria and is involved in the exchange of aspartate for glutamate across the inner mitochondrial membrane. Polymorphisms in this gene may be associated with autism, and mutations in this gene may also be a cause of global cerebral hypomyelination. Alternatively spliced transcript variants have been observed for this gene.Immunogen information
Immunogen Recombinant protein of Human SLC25A12. Sequence Email for sequence Gene ID Swiss Prot Synonyms AGC1; DEE39; ARALAR; EIEE39 Calculated MW 75kDa Observed MW 70kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IP Recommended dilution - WB 1:500 - 1:1000
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples U-251MG, U-2 OS, Mouse brain, Mouse testis, Mouse skeletal muscle, Rat brain, Rat skeletal muscle, Rat heart Cellular location Mitochondrion inner membrane, Multi-pass membrane protein 
Western blot analysis of extracts of various cell lines, using SLC25A12 antibody (A21129) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot analysis of extracts of various cell lines, using SLC25A12 antibody (A21129) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunoprecipitation analysis of 600 μg extracts of Mouse brain using 3 μg SLC25A12 antibody (A21129). Western blot was performed from the immunoprecipitate using SLC25A12 antibody (A21129) at a dilution of1:1000.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of Human SLC25A12. Isotype IgG