быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
SNX4 Rabbit mAb (A21130)
- Описание
- Характеристики
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Overview
Product name SNX4 Rabbit mAb Catalog No. A21130 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3028 Background
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associated with the long isoform of the leptin receptor and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor in cell cultures, but its function is unknown. This protein may form oligomeric complexes with family members. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.Immunogen information
Immunogen Recombinant protein of Human SNX4. Sequence Email for sequence Gene ID Swiss Prot Synonyms ATG24B Calculated MW 52kDa Observed MW 58kDa Applications
Reactivity Human, Mouse Tested applications WB IP Recommended dilution - WB 1:500 - 1:1000
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples HeLa, Jurkat, NIH/3T3, Mouse heart Cellular location Cytoplasmic side, Early endosome membrane, Peripheral membrane protein 
Western blot analysis of various lysates, using SNX4 Rabbit mAb (A21130) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg SNX4 antibody (A21130). Western blot was performed from the immunoprecipitate using SNX4 antibody (A21130) at a dilution of1:1000.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant protein of Human SNX4. Isotype IgG