быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
NMI Rabbit mAb (A21131)
- Описание
- Характеристики
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Overview
Product name NMI Rabbit mAb Catalog No. A21131 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3069 Background
NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.Immunogen information
Immunogen Recombinant protein of Human NMI. Sequence Email for sequence Gene ID Swiss Prot Synonyms NMI Calculated MW 35kDa Observed MW 38kDa Applications
Reactivity Human Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples HeLa, A-431, K-562 Cellular location Cytoplasm 
Western blot analysis of extracts of various cell lines, using NMI antibody (A21131) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human tonsil using NMI Rabbit mAb (A21131) at dilution of 1:50(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of Human NMI. Isotype IgG