быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
PWP1 Rabbit mAb (A21143)
- Описание
- Характеристики
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Overview
Product name PWP1 Rabbit mAb Catalog No. A21143 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3017 Background
The protein encoded by this gene contains several WD-40 repeats and is found mostly in the nucleus. The expression and localization of this protein are cell cycle dependent. Expression of this gene is upregulated in pancreatic adenocarcinoma. Three transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of Human PWP1. Sequence Email for sequence Gene ID Swiss Prot Synonyms NCLB; IEF-SSP-9502 Calculated MW 56kDa Observed MW 75kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, Jurkat, Mouse liver, Rat thymus Cellular location Nucleus 
Western blot analysis of extracts of various cell lines, using PWP1 antibody (A21143) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using PWP1 antibody (A21143) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of Human PWP1. Isotype IgG