быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
PMF1 Rabbit mAb (A21144)
- Описание
- Характеристики
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Overview
Product name PMF1 Rabbit mAb Catalog No. A21144 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3037 Background
Enables leucine zipper domain binding activity and transcription coactivator activity. Involved in chromosome segregation. Located in Golgi apparatus; kinetochore; and nucleoplasm. Part of MIS12/MIND type complex. Implicated in bladder carcinoma and urinary bladder cancer.Immunogen information
Immunogen Recombinant protein of Human PMF1. Sequence Email for sequence Gene ID Swiss Prot Synonyms PMF1 Calculated MW 23kDa Observed MW 23kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples MCF7, Jurkat, HeLa, HepG2, Mouse lung, Mouse brain, Rat testis Cellular location cytosol, Golgi apparatus, nucleoplasm 
Western blot analysis of extracts of various cell lines, using PMF1 antibody (A21144) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using PMF1 antibody (A21144) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of Human PMF1. Isotype IgG