быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
CD147 Rabbit mAb (A21147)
- Описание
- Характеристики
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Overview
Product name CD147 Rabbit mAb Catalog No. A21147 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3054 Background
Predicted to enable cell-cell adhesion mediator activity; signaling receptor activity; and virus receptor activity. Involved in neural retina development; photoreceptor cell maintenance; and spermatogenesis. Located in several cellular components, including acrosomal membrane; photoreceptor inner segment; and photoreceptor outer segment. Is expressed in several structures, including alimentary system; brain; early conceptus; reproductive system; and sensory organ. Orthologous to human BSG (basigin (Ok blood group)).Immunogen information
Immunogen Recombinant protein of Mouse CD147. Sequence Email for sequence Gene ID Swiss Prot Synonyms HT-7; CD147; EMMPRIN Calculated MW 42kDa Observed MW 38-58kDa Applications
Reactivity Mouse Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Mouse lung, Mouse liver, Mouse heart Cellular location basolateral plasma membrane, endosome, mitochondrion, plasma membrane 
Western blot analysis of extracts of various cell lines, using CD147 antibody (A21147) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 25s.
Immunohistochemistry analysis of paraffin-embedded mouse liver using CD147 Rabbit mAb (A21147) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse lung using CD147 Rabbit mAb (A21147) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse stomach using CD147 Rabbit mAb (A21147) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Мышь Immunogen Recombinant protein of Mouse CD147. Isotype IgG