быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
NSDHL Rabbit mAb (A21152)
- Описание
- Характеристики
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Overview
Product name NSDHL Rabbit mAb Catalog No. A21152 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3040 Background
The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene.Immunogen information
Immunogen Recombinant protein of Human NSDHL. Sequence Email for sequence Gene ID Swiss Prot Synonyms H105E3; XAP104; SDR31E1 Calculated MW 42kDa Observed MW 40kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, A-431 Cellular location endoplasmic reticulum, lipid droplet 
Western blot analysis of extracts of various cell lines, using NSDHL antibody (A21152) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of Human NSDHL. Isotype IgG