быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
EMC4 Rabbit mAb (A21153)
- Описание
- Характеристики
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Overview
Product name EMC4 Rabbit mAb Catalog No. A21153 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3006 Background
Contributes to membrane insertase activity. Involved in protein insertion into ER membrane by stop-transfer membrane-anchor sequence and tail-anchored membrane protein insertion into ER membrane. Is integral component of endoplasmic reticulum membrane. Part of EMC complex.Immunogen information
Immunogen Recombinant protein of Human EMC4. Sequence Email for sequence Gene ID Swiss Prot Synonyms PIG17; TMEM85 Calculated MW 15kDa/16kDa/20kDa Observed MW 20kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:1000 - 1:5000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples 293T, Hep G2, SH-SY5Y, U-87 MG, Mouse brain, Mouse heart, Rat brain, Rat heart Cellular location Endoplasmic reticulum membrane 
Western blot analysis of extracts of various cell lines, using EMC4 antibody (A21153) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of extracts of various cell lines, using EMC4 antibody (A21153) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human brain using EMC4 Rabbit mAb (A21153) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using EMC4 Rabbit mAb (A21153) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using EMC4 Rabbit mAb (A21153) at dilution of 1:100(40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of Human EMC4. Isotype IgG