быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
DNAJA4 Rabbit mAb (A21155)
- Описание
- Характеристики
-
Overview
Product name DNAJA4 Rabbit mAb Catalog No. A21155 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC3009 Background
Enables chaperone binding activity and unfolded protein binding activity. Involved in several processes, including negative regulation of endothelial cell migration; negative regulation of inclusion body assembly; and protein refolding. Located in cytosol and membrane.Immunogen information
Immunogen Recombinant protein of Human DNAJA4. Sequence Email for sequence Gene ID Swiss Prot Synonyms MST104; MSTP104; PRO1472 Calculated MW 45kDa Observed MW 45kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IP Recommended dilution - WB 1:500 - 1:1000
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples Mouse heart, Rat heart, Mouse brain Cellular location cytoplasm, cytosol 
Western blot analysis of extracts of various cell lines, using DNAJA4 antibody (A21155) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts of THP-1 cells, using DNAJA4 antibody (A21155) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Immunoprecipitation analysis of 600 μg extracts of Mouse brain using 3 μg DNAJA4 antibody (A21155). Western blot was performed from the immunoprecipitate using DNAJA4 antibody (A21155) at a dilution of1:1000.
-
Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of Human DNAJA4. Isotype IgG